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AFF4 Polyklonaler Antikörper

AFF4 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14662-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using AFF4 Polyclonal antibody (14662-1-AP)

    WB analysis using 14662-1-AP (same clone as 14662-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14662-1-AP (AFF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14662-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using AFF4 Polyclonal antibody (14662-1-AP)

    IP experiment of HeLa using 14662-1-AP (same clone as 14662-1-PBS)

    IP result of anti-AFF4 (IP:14662-1-AP, 4ug; Detection:14662-1-AP 1:20000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 14662-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

14662-1-PBS bindet in WB, IP, Indirect ELISA AFF4 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen AFF4 fusion protein Ag6336
Vollständiger Name AF4/FMR2 family, member 4
Berechnetes Molekulargewicht 127 kDa
Beobachtetes Molekulargewicht 140-150 kDa
GenBank-ZugangsnummerBC063007
Gene symbol AFF4
Gene ID (NCBI) 27125
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transcriptional controled by RNA polymerase II (Pol II) is a multi-step process requiring the concerted action of multiple factors and contacts with the DNA template for the proper synthesis of nascent RNA [PMID:12676794]. ELL1 was demonstrated to be a Pol II elongation factor capable of enhancing the catalytic rate of transcription elongation by reducing transient pausing by the enzyme [PMID:8596958]. AFF4 was initially identified as a fusion partner of the MLL (mixed-lineage leukemia) gene participated in infant acute lymphoblastic leukemia, and a component of the ELL/p-TEFb elongation complex. AFF4 interacts with positive transcription elongation factor-b while also repressing Tat transactivation of HIV-1 [PMID:17389929].