AFMID Polyklonaler Antikörper

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• KD/KO Validated

AFMID Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 19522-1-AP

Synonyme

AFMID, arylformamidase, KF, Kynurenine formamidase, Probable arylformamidase

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  WB analysis of mouse kidney using 19522-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19522-1-AP (AFMID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 19522-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19522-1-AP (AFMID antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 19522-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 19522-1-AP (AFMID antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 19522-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19522-1-AP (AFMID antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 19522-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19522-1-AP (AFMID antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, humanes Lebergewebe, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

19522-1-AP bindet in WB, IHC, ELISA AFMID und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: arylformamidase
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: NM_001145526
• Gene symbol: AFMID
• Gene ID (NCBI): 125061
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AFMID,also named as KF, belongs to the AFMID family. AFMID catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the conversion of tryptophan to nicotinic acid, NAD(H) and NADP(H). AFMID is required for elimination of toxic metabolites.

Publikationen

Species	Application	Title
human, mouse	WB	Cancer Cell; Inhibition of De Novo NAD(+) Synthesis by Oncogenic URI Causes Liver Tumorigenesis through DNA Damage.; Authors - Krishna S Tummala ; KD Validated
human	WB	Genome Res; A human-specific switch of alternatively spliced AFMID isoforms contributes to TP53 mutations and tumor recurrence in hepatocellular carcinoma.; Authors - Kuan-Ting Lin
mouse	WB	Mol Cell Endocrinol; Hepatic NAD+levels and NAMPT abundance are unaffected during prolonged high-fat diet consumption in C57BL/6JBomTac mice.; Authors - Morten Dall
human	WB	J Proteome Res; Proteomics Analysis of Renal Cell Line Caki-2 with AFMID Overexpression and Potential Biomarker Discovery in Urine; Authors - Jiameng Sun