AFP Polyklonaler Antikörper

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AFP Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14550-1-PBS

Synonyme

HPAFP, Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein

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  WB analysis using 14550-1-AP (same clone as 14550-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14550-1-AP (AFP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14550-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 14550-1-AP (same clone as 14550-1-PBS)

  WB result of AFP antibody (14550-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-AFP transfected HepG2 cells. This data was developed using the same antibody clone with 14550-1-PBS in a different storage buffer formulation.

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  WB analysis of BxPC-3 using 14550-1-AP (same clone as 14550-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14550-1-AP (AFP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14550-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 14550-1-AP (same clone as 14550-1-PBS)

  IP result of anti-AFP (IP:14550-1-AP, 5ug; Detection:14550-1-AP 1:1000) with HepG2 cells lysate 6000ug. This data was developed using the same antibody clone with 14550-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 14550-1-AP (same clone as 14550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 14550-1-AP (AFP antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14550-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 14550-1-AP (same clone as 14550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14550-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14550-1-AP (same clone as 14550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14550-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14550-1-AP (same clone as 14550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14550-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14550-1-AP (same clone as 14550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14550-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 14550-1-AP (same clone as 14550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14550-1-AP (AFP Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14550-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 14550-1-AP (same clone as 14550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14550-1-AP (AFP Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14550-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14550-1-PBS bindet in WB, IHC, IP, Indirect ELISA AFP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AFP fusion protein Ag6089
• Vollständiger Name: alpha-fetoprotein
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 68-72 kDa
• GenBank-Zugangsnummer: BC027881
• Gene symbol: AFP
• Gene ID (NCBI): 174
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AFP (Alpha-fetoprotein) is a major plasma protein in the fetus and its concentration is very low in the adult (PMID:24120489). AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma, indicating that AFP can serve as a tumor marker (PMID: 18669658). AFP is also a glycosylated protein and based on its binding capability to lectin Lens Culinaris Agglutinin (LCA), and total AFP can be separated into three different glycoforms, AFP-L1, AFP-L2, and AFP-L3. Core-fucosylated form of AFP (AFP-L3) is a more specific indicator than total AFP for HCC (PMID: 33128033, 35458505)