AFP Monoklonaler Antikörper

AFP Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4B3E4

Kat-Nr. : 67852-1-PBS

Synonyme

4B3E4, Alpha fetoglobulin, alpha fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin

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  WB analysis of HepG2 using 67852-1-Ig (same clone as 67852-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

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  WB analysis of HuH-7 using 67852-1-Ig (same clone as 67852-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 67852-1-Ig (same clone as 67852-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67852-1-Ig (same clone as 67852-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67852-1-Ig (same clone as 67852-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67852-1-Ig (AFP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67852-1-Ig (same clone as 67852-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67852-1-Ig (AFP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67852-1-Ig (same clone as 67852-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67852-1-Ig (AFP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 67852-1-Ig (same clone as 67852-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67852-1-Ig (AFP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67852-1-PBS bindet in WB, IHC, Indirect ELISA AFP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AFP fusion protein Ag6089
• Vollständiger Name: alpha-fetoprotein
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 68-72 kDa
• GenBank-Zugangsnummer: BC027881
• Gene symbol: AFP
• Gene ID (NCBI): 174
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AFP (Alpha-fetoprotein) is a major plasma protein in the fetus and its concentration is very low in the adult (PMID:24120489). AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma, indicating that AFP can serve as a tumor marker (PMID: 18669658). AFP is also a glycosylated protein and based on its binding capability to lectin Lens Culinaris Agglutinin (LCA), and total AFP can be separated into three different glycoforms, AFP-L1, AFP-L2, and AFP-L3. Core-fucosylated form of AFP (AFP-L3) is a more specific indicator than total AFP for HCC (PMID: 33128033, 35458505)