AFP Rekombinanter Antikörper

AFP Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

230435G1

Kat-Nr. : 82982-3-PBS

Synonyme

Alpha fetoglobulin, alpha fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein

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  Sandwich ELISA standard curve of MP00001-4

  Sandwich ELISA standard curve of MP00001-4, AFP Recombinant Matched Antibody Pair - PBS only. 82982-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0539. 82982-4-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

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  Sample test of MP00001-4

  Serum of sixteen individual healthy human donors was measured. The AFP concentration of detected samples was determined to be 8,740.7 pg/mL with a range of 2,872.1 - 32,900 pg/mL.

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  Sample test of MP00001-4

  HepG2 (human hepatocellular carcinoma cells) were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The mean AFP concentration was determined to be 3,038.3 ng/mL in HepG2 supernatant.

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Geprüfte Anwendungen

Produktinformation

82982-3-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test AFP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: AFP fusion protein Eg0539
• Vollständiger Name: alpha-fetoprotein
• Berechnetes Molekulargewicht: 69 kDa
• GenBank-Zugangsnummer: BC027881
• Gene symbol: AFP
• Gene ID (NCBI): 174
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.