• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

AFP Rekombinanter Antikörper

AFP Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230435E3

Kat-Nr. : 82982-6-PBS

Synonyme

Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, Alpha fetoglobulin

Filter:
  • Western Blot (WB) analysis of human placenta tissue using AFP Recombinant antibody (82982-6-RR)

    WB analysis of human placenta using 82982-6-RR (same clone as 82982-6-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 82982-6-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82982-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using AFP Recombinant antibody (82982-6-RR)

    IHC staining of human liver using 82982-6-RR (same clone as 82982-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82982-6-RR (AFP antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82982-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using AFP Recombinant antibody (82982-6-RR)

    IHC staining of human liver using 82982-6-RR (same clone as 82982-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82982-6-RR (AFP antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82982-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82982-6-RR

    Affinity and Kinetic Characterization of 82982-6-RR

    Biolayer interferometry (BLl) kinetic assays of 82982-6-RR against Human AFP were performed. The affinity constant is 2.80 nM.

Geprüfte Anwendungen

Produktinformation

82982-6-PBS bindet in WB, IHC, Indirect ELISA AFP und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Fusionsprotein
Vollständiger Name alpha-fetoprotein
Berechnetes Molekulargewicht 69 kDa
Beobachtetes Molekulargewicht68 kDa
GenBank-ZugangsnummerBC027881
Gene symbol AFP
Gene ID (NCBI) 174
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AFP (Alpha-fetoprotein) is a major plasma protein in the fetus and its concentration is very low in the adult (PMID:24120489). AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma, indicating that AFP can serve as a tumor marker (PMID: 18669658). AFP is also a glycosylated protein and based on its binding capability to lectin Lens Culinaris Agglutinin (LCA), and total AFP can be separated into three different glycoforms, AFP-L1, AFP-L2, and AFP-L3. Core-fucosylated form of AFP (AFP-L3) is a more specific indicator than total AFP for HCC (PMID: 33128033, 35458505)