AGA Polyklonaler Antikörper

AGA Polyklonal Antikörper für IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17299-1-PBS

Synonyme

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  IHC staining of mouse kidney using 17299-1-AP (same clone as 17299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17299-1-AP (AGA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17299-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 17299-1-AP (same clone as 17299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17299-1-AP (AGA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17299-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 17299-1-AP (same clone as 17299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17299-1-AP (AGA Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17299-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 17299-1-AP (same clone as 17299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17299-1-AP (AGA Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17299-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17299-1-PBS bindet in IHC, Indirect ELISA AGA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AGA fusion protein Ag10681
• Vollständiger Name: aspartylglucosaminidase
• Berechnetes Molekulargewicht: 346 aa, 37 kDa
• GenBank-Zugangsnummer: BC012392
• Gene symbol: AGA
• Gene ID (NCBI): 175
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AGA(Aspartylglucosaminidase) is also named as N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase and glycosylasparaginase.The open reading frame of the readthrough transcript would indicate AGA close to 40 kDa.It can be cleaved into 2 chains:the 24 kDa α- and 17 kDa β-subunits of the wild-type AGA,in addition,an aberrant polypeptide with a mol.wt of 21 kDa is also detected as a product of proteolytic cleavage of the mutant precursor molecule(PMID: 9425233).AGA can exsit as a dimer with the mol.wt between 100 kDa and 49 kDa and His124 at an interface between two heterodimers of AGA is crucial for the thermodynamically stable oligomeric structure of AGA.(PMID:9737998). It can also exsit as a heterotetrameric protein of 88 kDa(PMID:1733831).