AGAP2 Polyklonaler Antikörper

AGAP2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14690-1-PBS

Synonyme

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  WB analysis of human brain using 14690-1-AP (same clone as 14690-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14690-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 14690-1-AP (same clone as 14690-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14690-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 14690-1-AP (same clone as 14690-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14690-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 14690-1-AP (same clone as 14690-1-PBS)

  IP result of anti-AGAP2 (IP:14690-1-AP, 4ug; Detection:14690-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 14690-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 14690-1-AP (same clone as 14690-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14690-1-AP (AGAP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14690-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 14690-1-AP (same clone as 14690-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14690-1-AP (AGAP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14690-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14690-1-AP (same clone as 14690-1-PBS)

  Immunofluorescent analysis of Hela cells, using AGAP2 antibody 14690-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14690-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14690-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA AGAP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AGAP2 fusion protein Ag6142
• Vollständiger Name: ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
• Berechnetes Molekulargewicht: 125 kDa
• Beobachtetes Molekulargewicht: 124 kDa
• GenBank-Zugangsnummer: BC028020
• Gene symbol: AGAP2
• Gene ID (NCBI): 116986
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.