AGER/RAGE Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

AGER/RAGE Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(51)

Kat-Nr. : 16346-1-AP

Synonyme

RAGE, AGER, Advanced glycosylation end product-specific receptor

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  WB analysis using 16346-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16346-1-AP (AGER/RAGE antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 16346-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16346-1-AP (AGER/RAGE antibody) at dilution of 1:4000 (under 10x lens).

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  IHC staining of mouse lung using 16346-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16346-1-AP (AGER/RAGE antibody) at dilution of 1:4000 (under 40x lens).

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  IHC staining of human lung using 16346-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 16346-1-AP (AGER antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

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  IHC staining of human renal cell carcinoma using 16346-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 16346-1-AP (AGER Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human renal cell carcinoma using 16346-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 16346-1-AP (AGER Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung using 16346-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 16346-1-AP (AGER antibody) at dilution of 1:4000 (under 10x lens).

• 

  IF Staining of mouse lung using 16346-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using AGER/RAGE antibody (16346-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, Rattenlungengewebe
• Erfolgreiche Detektion in IHC: Mauslungengewebe, humanes Lungengewebe, humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mauslungengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 38 publications below
• IHC: See 10 publications below
• IF: See 11 publications below

Produktinformation

16346-1-AP bindet in WB, IHC, IF-P, ELISA AGER/RAGE und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AGER/RAGE fusion protein Ag9540
• Vollständiger Name: advanced glycosylation end product-specific receptor
• Berechnetes Molekulargewicht: 404 aa, 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC020669
• Gene symbol: AGER
• Gene ID (NCBI): 177
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a member of the immunoglobulin superfamily of cell surface receptors, which interacts with distinct families of ligands, mediating diverse functions in a broad array of cell types including cellular migration, proliferation, survival and apoptosis (PMID: 12645002; 17425919). It senses endogenous stress signals with a broad ligand repertoire including advanced glycation end products, S100 proteins, high-mobility group box 1 protein/HMGB1, amyloid beta/APP oligomers, nucleic acids, phospholipids and glycosaminoglycans (PMID: 19910580; 28627626). It interacts with distinct molecules implicated in homeostasis, development, inflammation, and certain diseases such as diabetes and Alzheimer's disease (PMID: 26253613; 31079281).

Publikationen

Species	Application	Title
	IF	EMBO J; A novel human fetal lung-derived alveolar organoid model reveals mechanisms of surfactant protein C maturation relevant to interstitial lung disease; Authors - Kyungtae Lim
Rat		Oxid Med Cell Longev; l-Theanine Ameliorates d-Galactose-Induced Brain Damage in Rats via Inhibiting AGE Formation and Regulating Sirtuin1 and BDNF Signaling Pathways.; Authors - Li Zeng
rat	IF,WB	J Neuroinflammation; Inhibiting HMGB1-RAGE axis prevents pro-inflammatory macrophages/microglia polarization and affords neuroprotection after spinal cord injury.; Authors - Hong Fan
mouse	WB	J Neuroinflammation; Celastrol exerts a neuroprotective effect by directly binding to HMGB1 protein in cerebral ischemia-reperfusion.; Authors - Dan-Dan Liu
human,mouse	WB,IHC	Mol Med; AGEs promote atherosclerosis by increasing LDL transcytosis across endothelial cells via RAGE/NF-κB/Caveolin-1 pathway; Authors - Meng Shu ; KD Validated
mouse	WB	Front Pharmacol; Protective effect of heat-processed Gynostemma pentaphyllum on high fat diet-induced glucose metabolic disorders mice; Authors - Jin-Bo Xie