AGER/RAGE Rekombinanter Antikörper

AGER/RAGE Rekombinant Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

WB, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

240706F7

Kat-Nr. : 83759-4-RR

Synonyme

RAGE, AGER, Advanced glycosylation end product-specific receptor, 240706F7

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  WB analysis of rat lung using 83759-4-RR

  rat lung tissue were subjected to SDS PAGE followed by western blot with 83759-4-RR (RAGE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of rat brain using 83759-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using AGER antibody (83759-4-RR, Clone: 240706F7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 83759-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using AGER antibody (83759-4-RR, Clone: 240706F7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83759-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83759-4-RR against Mouse AGER were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenlungengewebe
• Erfolgreiche Detektion in IF-P: Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83759-4-RR bindet in WB, IF-P, ELISA AGER/RAGE und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: advanced glycosylation end product-specific receptor
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 45 kDa, 58 kDa
• Gene symbol: Ager
• Gene ID (NCBI): 81722
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a member of the immunoglobulin superfamily of cell surface receptors, which interacts with distinct families of ligands, mediating diverse functions in a broad array of cell types including cellular migration, proliferation, survival and apoptosis (PMID: 12645002; 17425919). It senses endogenous stress signals with a broad ligand repertoire including advanced glycation end products, S100 proteins, high-mobility group box 1 protein/HMGB1, amyloid beta/APP oligomers, nucleic acids, phospholipids and glycosaminoglycans (PMID: 19910580; 28627626). It interacts with distinct molecules implicated in homeostasis, development, inflammation, and certain diseases such as diabetes and Alzheimer's disease (PMID: 26253613; 31079281).