AGER/RAGE Monoklonaler Antikörper

AGER/RAGE Monoklonal Antikörper für WB, IF-P

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF-P

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3D4G11

Kat-Nr. : CL488-66833

Synonyme

AGER, RAGE, 3D4G11, Advanced glycosylation end product-specific receptor

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  WB analysis using CL488-66833

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66833 (AGER antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse lung using CL488-66833

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using CoraLite® Plus 488 AGER/RAGE antibody (CL488-66833, Clone: 3D4G11 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse lung using CL488-66833

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using CoraLite® Plus 488 AGER/RAGE antibody (CL488-66833, Clone: 3D4G11 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat lung using CL488-66833

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat lung tissue using CoraLite® Plus 488 AGER antibody (CL488-66833, Clone: 3D4G11 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Lungengewebe
• Erfolgreiche Detektion in IF-P: Rattenlungengewebe, Mauslungengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66833 bindet in WB, IF-P AGER/RAGE und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AGER/RAGE fusion protein Ag9657
• Vollständiger Name: advanced glycosylation end product-specific receptor
• Berechnetes Molekulargewicht: 404 aa, 43 kDa
• Beobachtetes Molekulargewicht: 43, 50 kDa
• GenBank-Zugangsnummer: BC020669
• Gene symbol: AGER
• Gene ID (NCBI): 177
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a multiligand receptor of the immunoglobulin superfamily. It interacts with distinct molecules implicated in homeostasis, development, and inflammation, and certain diseases such as diabetes and Alzheimer disease.