AGFG1 Polyklonaler Antikörper

AGFG1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 12670-1-AP

Synonyme

AGFG1, ArfGAP with FG repeats 1, HIV 1 Rev binding protein, HRB, Nucleoporin like protein RIP, RAB, Rev interacting protein, RIP

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Galerie der Validierungsdaten

• 

  WB analysis of mouse testis using 12670-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12670-1-AP (AGFG1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12670-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12670-1-AP (AGFG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12670-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12670-1-AP (AGFG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 12670-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 12670-1-AP (AGFG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12670-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12670-1-AP (AGFG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12670-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12670-1-AP (AGFG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12670-1-AP

  IP Result of anti-AGFG1 (IP:12670-1-AP, 4ug; Detection:12670-1-AP 1:400) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human breast cancer using 12670-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12670-1-AP (AGFG1 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, HeLa-Zellen, HepG2-Zellen, HT-1080.Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

12670-1-AP bindet in WB, IP, IHC, IF, ELISA AGFG1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AGFG1 fusion protein Ag3352
• Vollständiger Name: ArfGAP with FG repeats 1
• Berechnetes Molekulargewicht: 562 aa, 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC030592
• Gene symbol: AGFG1
• Gene ID (NCBI): 3267
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IF	Cell; A Translation-Activating Function of MIWI/piRNA during Mouse Spermiogenesis.; Authors - Peng Dai
		Proc Natl Acad Sci U S A; Biogenesis of sperm acrosome is regulated by pre-mRNA alternative splicing of Acrbp in the mouse.; Authors - Yoshinori Kanemori