AGGF1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

AGGF1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

20

Kat-Nr. : 11889-1-AP

Synonyme

AGGF1, Angiogenic factor VG5Q, GPATC7, GPATCH7, HSU84971, HUS84971, hVG5Q, VG5Q

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 11889-1-AP

  WB result of AGGF1 antibody (11889-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AGGF1 transfected HeLa cells.

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  WB analysis using 11889-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11889-1-AP (AGGF1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, HeLa-Zellen, L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 16 publications below
• IHC: See 7 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Produktinformation

11889-1-AP bindet in WB, IP, IHC, IF, ELISA AGGF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rhesusaffe, Makake
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AGGF1 fusion protein Ag2497
• Vollständiger Name: angiogenic factor with G patch and FHA domains 1
• Berechnetes Molekulargewicht: 714 aa, 81 kDa
• Beobachtetes Molekulargewicht: 84-100 kDa
• GenBank-Zugangsnummer: BC029382
• Gene symbol: AGGF1
• Gene ID (NCBI): 55109
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The angiogenic factor gene, AGGF1 (or VG5Q), is identified as a candidate susceptibility gene for Klippel-Trenaunay syndrome (KTS) which is a severe congenital disorder characterized by capillary malformations, venous malformations or varicose veins, and hypertrophy of the affected tissues. AGGF1 protein can bind to endothelial cells and promote cell proliferation. AGGF1 shows strong expression in blood vessels and is secreted as vessel formation is initiated. Regulation of AGGF1 by GATA1 may play roles in endothelial cell biology and angiogenesis. Catalog#11889-1-AP was validated by KO and KD methods in scientists' studies (PMID: 28153879; 27513923), and the observed MW is 84 kDa.

Publikationen

Species	Application	Title
human	WB,IHC	Nat Commun; A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure.; Authors - Yufeng Yao
mouse	WB	Mol Ther; The 3' Untranslated Region Protects the Heart from Angiotensin II-Induced Cardiac Dysfunction via AGGF1 Expression.; Authors - Lexi Ding
mouse	WB	PLoS Biol; Angiogenic Factor AGGF1 Activates Autophagy with an Essential Role in Therapeutic Angiogenesis for Heart Disease.; Authors - Qiulun Lu ; KO Validated
rat	WB,IF	Arterioscler. Thromb. Vasc. Biol.; Angiogenic Factor With G Patch and FHA Domains 1 Is a Novel Regulator of Vascular Injury.; Authors - Bisheng Zhou ; KD Validated
human	WB	Mol Ther Nucleic Acids; circ-SHKBP1 Regulates the Angiogenesis of U87 Glioma-Exposed Endothelial Cells through miR-544a/FOXP1 and miR-379/FOXP2 Pathways.; Authors - Qianru He
		FASEB J; Angiotensin II increases angiogenesis by NF-κB-mediated transcriptional activation of angiogenic factor AGGF1.; Authors - Wenxia Si

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