AGPAT2 Rekombinanter Antikörper

AGPAT2 Rekombinant Antikörper für WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240153C2

Kat-Nr. : 83349-3-PBS

Synonyme

1 AGPAT2, 1-acylglycerol-3-phosphate O-acyltransferase 2, 1-acyl-sn-glycerol-3-phosphate acyltransferase beta, 1-AGP acyltransferase 2, 1-AGPAT 2

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  WB analysis using 83349-3-RR (same clone as 83349-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83349-3-RR (AGPAT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83349-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00360-4

  Sandwich ELISA standard curve of MP00360-4, Human AGPAT2 Recombinant Matched Antibody Pair - PBS only. 83349-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34761. 83349-4-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

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  Sample test of MP00360-4

  The mean AGPAT2 concentration was determined to be 2.20 ng/mL in HeLa cell extract based on a 2.0 mg/mL extract load and 23.90 ng/mL in THP-1 cell extract based on a 2.0 mg/mL extract load.

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  IHC staining of human ovary cancer using 83349-3-RR (same clone as 83349-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83349-3-RR (AGPAT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83349-3-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 83349-3-RR (same clone as 83349-3-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug AGPAT2 Recombinant antibody (83349-3-RR, Clone:240153C2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83349-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00360-2

  Cytometric bead array standard curve of MP00360-2, AGPAT2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83349-3-PBS. Detection antibody: 83349-1-PBS. Standard: Ag34761. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83349-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83349-3-RR against Human AGPAT2 were performed. The affinity constant is 2.72 nM.

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Geprüfte Anwendungen

Produktinformation

83349-3-PBS bindet in WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test AGPAT2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: AGPAT2 fusion protein Ag34761
• Vollständiger Name: 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta)
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC019292
• Gene symbol: AGPAT2
• Gene ID (NCBI): 10555
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

1-acyl-sn-glycerol-3-phosphate acyltransferase beta (AGPAT2) belongs to a family of enzymes catalyzing the sn-2 acylation of the glycerol-3-phosphate backbone. AGPAT2 is highly expressed in adipose tissues, liver, and skeletal muscle. AGPAT2 is the only AGPAT isoform whose loss-of-function mutations cause a severe form of human congenital generalized lipodystrophy (PMID: 34824276). Human and mouse AGPAT2 have a calculated molecular mass of 31 kDa, and an alternatively spliced form of AGPAT2 mRNA, encoding a protein of 246 rather than 278 amino acids, is also found in human (PMID: 19336658). Western detected AGPAT2 at an apparent molecular mass of 27 kDa.