AGPAT4 Polyklonaler Antikörper

AGPAT4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16562-1-AP

Synonyme

1 AGP acyltransferase 4, 1 AGPAT 4, 1 AGPAT4, 1-acylglycerol-3-phosphate O-acyltransferase 4, 1-acyl-sn-glycerol-3-phosphate acyltransferase delta

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  WB analysis using 16562-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16562-1-AP (AGPAT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16562-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16562-1-AP (AGPAT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 16562-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16562-1-AP (AGPAT4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16562-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16562-1-AP (AGPAT4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, U-251-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16562-1-AP bindet in WB, IHC, ELISA AGPAT4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AGPAT4 fusion protein Ag9834
• Vollständiger Name: 1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphatidic acid acyltransferase, delta)
• Berechnetes Molekulargewicht: 378 aa, 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC020209
• Gene symbol: AGPAT4
• Gene ID (NCBI): 56895
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AGPAT4 (Acylglycerophosphate acyltransferase 4), also known as LPAATδ (Lysophosphatidic acid acyltransferase delta), is a protein expressed mainly in brain and in muscle. AGPAT4 is characterized as a mitochondrial lysophosphatidic acid acyltransferase that regulates brain levels of phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylinositol (PI) (PMID: 28807933). AGPAT4 is relatively abundant in murine renal white adipose tissue (WAT) and has been linked to the regulation of TAG production, the differentiation of adipocytes, and the modulation of various pathologies including lipodystrophy, diabetes, and hepatic steatosis (PMID: 28814640).