AGPAT6 Polyklonaler Antikörper

AGPAT6 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 16762-1-AP

Synonyme

1 AGP acyltransferase 6, 1 AGPAT 6, AGPAT6, GPAT4, LPAAT zeta, LPAATZ, TSARG7

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  WB analysis using 16762-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16762-1-AP (AGPAT6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 16762-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16762-1-AP (AGPAT6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16762-1-AP

  IP result of anti-AGPAT6 (IP:16762-1-AP, 4ug; Detection:16762-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

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  IHC staining of human testis using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maushodengewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Nierengewebe, humanes Lebergewebe, humanes Eierstockgewebe, humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

16762-1-AP bindet in WB, IP, IHC, ELISA AGPAT6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AGPAT6 fusion protein Ag10294
• Vollständiger Name: 1-acylglycerol-3-phosphate O-acyltransferase 6 (lysophosphatidic acid acyltransferase, zeta)
• Berechnetes Molekulargewicht: 456 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC051377
• Gene symbol: AGPAT6
• Gene ID (NCBI): 137964
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AGPAT6 is a member of the 1-acylglycerol-3-phosphate O-acyltransferase (AGPAT) family. AGPAT6 is expressed in brown adipose tissue and mammary epithelium as well as many other tissues. A deficiency of AGPAT6 in mice results in resistance to dietinduced and genetic forms of obesity.

Publikationen

Species	Application	Title
human	WB	Invest Ophthalmol Vis Sci; Alteration in Lysophospholipids and Converting Enzymes in Glaucomatous Optic Nerves.; Authors - Sasha M Milbeck
mouse	WB	EMBO Rep; MTCH2 cooperates with MFN2 and lysophosphatidic acid synthesis to sustain mitochondrial fusion; Authors - Andres Goldman