AGR2 Polyklonaler Antikörper

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• KD/KO Validated

AGR2 Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 12275-1-AP

Synonyme

AG 2, AG2, AGR2, GOB 4, HAG 2, HPC8, PDIA17, XAG 2

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Galerie der Validierungsdaten

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  WB analysis using 12275-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat colon using 12275-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12275-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 12275-1-AP

  IP result of anti-AGR2(IP:12275-1-AP, 4ug; Detection:12275-1-AP 1:1000) with A549 cells lysate 1200 ug.

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  IHC staining of human breast cancer using 12275-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12275-1-AP (AGR2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12275-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12275-1-AP (AGR2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 12275-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 12275-1-AP (AGR2 Antibody) at dilution of 1:50.

• 

  IHC staining of human colon using 12275-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 12275-1-AP (AGR2 Antibody) at dilution of 1:50.

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  FC experiment of MCF-7 using 12275-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug AGR2 antibody (12275-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Maus-Magengewebe, Ratten-Kolongewebe, Ratten-Magengewebe
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 8 publications below
• IF: See 2 publications below

Produktinformation

12275-1-AP bindet in WB, IP, IHC, IF, FC, ELISA AGR2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AGR2 fusion protein Ag2919
• Vollständiger Name: anterior gradient homolog 2 (Xenopus laevis)
• Berechnetes Molekulargewicht: 175 aa, 20 kDa
• Beobachtetes Molekulargewicht: 17-20 kDa
• GenBank-Zugangsnummer: BC015503
• Gene symbol: AGR2
• Gene ID (NCBI): 10551
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AGR2, also named AG2 or HPC8, encodes anterior gradient protein 2 homolog which belongs to the AGR family. It is a secreted protein localized in endoplasmic reticulum. AGR2 plays roles in MUC2 post-transcriptional synthesis,secretion and production of mucus by intestinal cells. AGR2 was significantly elevated in the pancreatic juice from patients with pre-malignant conditions as well as pancreatic cancer compared to control pancreatic juice samples. AGR2 levels in pancreatic juice could potentially be used in assessment of high-risk patients undergoing endoscopic procedures.

Publikationen

Species	Application	Title
human	IF	Nat Cell Biol; Opposing Wnt signals regulate cervical squamocolumnar homeostasis and emergence of metaplasia.; Authors - Cindrilla Chumduri
human,mouse	IHC	Nat Protoc; Patient-derived and mouse endo-ectocervical organoid generation, genetic manipulation and applications to model infection.; Authors - Rajendra Kumar Gurumurthy
human	WB,IHC	Proc Natl Acad Sci U S A; A discrete population of squamocolumnar junction cells implicated in the pathogenesis of cervical cancer.; Authors - Herfs Michael M
human	IHC	J Pathol; A novel blueprint for 'top down' differentiation defines the cervical squamocolumnar junction during development, reproductive life, and neoplasia.; Authors - Michael Herfs
human	IHC	Int J Cancer; Unique recurrence patterns of cervical intraepithelial neoplasia following excision of the squamo-columnar junction.; Authors - Michael Herfs
human	WB	J Proteome Res; Identification of serum biomarkers for colorectal cancer metastasis using a differential secretome approach.; Authors - Xue Hua H

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