AGRN Polyklonaler Antikörper

AGRN Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20692-1-PBS

Synonyme

Agrin, Agrin C-terminal 110 kDa subunit, Agrin C-terminal 22 kDa fragment, Agrin C-terminal 90 kDa fragment, Agrin N-terminal 110 kDa subunit

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  WB analysis using 20692-1-AP (same clone as 20692-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20692-1-AP (AGRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20692-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 20692-1-AP (same clone as 20692-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20692-1-AP (AGRN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20692-1-PBS in a different storage buffer formulation.

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  IF Staining of Saos-2 using 20692-1-AP (same clone as 20692-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using AGRN antibody (20692-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20692-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20692-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA AGRN und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: agrin
• Berechnetes Molekulargewicht: 215 kDa
• Beobachtetes Molekulargewicht: 225 kDa, 97 kDa
• GenBank-Zugangsnummer: NM_198576
• Gene symbol: AGRN
• Gene ID (NCBI): 375790
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Agrin, a large heparan sulfate proteoglycan, is expressed in neuronal, as well as nonneuronal tissues (PMID:12671652). Nerve-derived agrin has been demonstrated to play an essential role in development and maintenance of the neuromuscular junction (PMID:16386415). The agrin gene encodes a protein of more than 2000 amino acids with predicted molecular weight of 225 kDa but can display as 600 kDa protein due to extensive N-terminal glycosylation (PMID:12671652). Neurotrypsin cleaves the proteoglycan agrin at two homologous, highly conserved sites, liberating a middle 90-kDa and a C-terminal 22-kDa fragment from the membrane or ECM-bound N-terminal moiety of agrin (PMID:18230682).