Angiotensinogen/AGT Polyklonaler Antikörper

Angiotensinogen/AGT Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 23972-1-AP

Synonyme

AGT, Angiotensinogen, Serpin A8, SERPINA8, Ang IV

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  WB analysis using 23972-1-AP

  Human plasma were subjected to SDS PAGE followed by western blot with 23972-1-AP (Angiotensinogen antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 23972-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23972-1-AP (Angiotensinogen antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 23972-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23972-1-AP (Angiotensinogen antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 23972-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 23972-1-AP (Angiotensinogen antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 23972-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 23972-1-AP (Angiotensinogen antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 23972-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23972-1-AP (Angiotensinogen antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 23972-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23972-1-AP (Angiotensinogen antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 23972-1-AP

  Immunofluorescent analysis of HepG2 cells using 23972-1-AP (Angiotensinogen antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of HEK-293 using 23972-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Angiotensinogen antibody (23972-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of L02 using 23972-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using Angiotensinogen antibody (23972-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  FC experiment of HeLa using 23972-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Angiotensinogen (23972-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human plasma
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Leberzirrhosegewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen, HepG2-Zellen, L02-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

23972-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Angiotensinogen/AGT und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Angiotensinogen/AGT fusion protein Ag21121
• Vollständiger Name: angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• Berechnetes Molekulargewicht: 485 aa, 53 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC011519
• Gene symbol: AGT
• Gene ID (NCBI): 183
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

Publikationen

Species	Application	Title
mouse	IF	JCI Insight; Osteopontin deficiency ameliorates Alport pathology by preventing tubular metabolic deficits.; Authors - Wen Ding