AGTR1 Polyklonaler Antikörper

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• KD/KO Validated

AGTR1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(70)

Rezensionen (2)

Kat-Nr. : 25343-1-AP

Synonyme

AG2S, AGTR1A, AGTR1B, Angiotensin II type 1 receptor, Angiotensin II type-1 receptor

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  WB analysis using 25343-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25343-1-AP (AGTR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 25343-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25343-1-AP (AGTR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 25343-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25343-1-AP (AGTR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 25343-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 25343-1-AP (AGTR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25343-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25343-1-AP (AGTR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 25343-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25343-1-AP (AGTR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 25343-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25343-1-AP (AGTR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 25343-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25343-1-AP (AGTR1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 25343-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25343-1-AP (AGTR1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 25343-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25343-1-AP (AGTR1 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C2C12-Zellen, HEK-293-Zellen, HuH-7-Zellen, Mausherzgewebe, Mausnierengewebe, PC-12-Zellen
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Herzgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 56 publications below
• IHC: See 17 publications below
• IF: See 14 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

25343-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA AGTR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AGTR1 fusion protein Ag14461
• Vollständiger Name: angiotensin II receptor, type 1
• Berechnetes Molekulargewicht: 359 aa, 41 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC022447
• Gene symbol: AGTR1
• Gene ID (NCBI): 185
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Angiotensin II (Ang II), the main effector molecule of the renin-angiotensin system, exerts its actions mainly via interaction with type-1 angiotensin II receptor (AGTR1, also named AT1R), thereby contributing to blood pressure regulation. AGTR1 mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. By regulating vascular tone, cardiovascular function, and salt and water homeostasis, AGTR1 exerts an indispensable physiological role (PMID: 21600887). AGTR1 has been implicated in diverse aspects of human disease, from the regulation of blood pressure and cardiovascular homeostasis to cancer progression (PMID: 26975580).

Publikationen

Species	Application	Title
mouse,human	WB,CoIP	Cell Res; Cartilage oligomeric matrix protein is an endogenous β-arrestin-2-selective allosteric modulator of AT1 receptor counteracting vascular injury.; Authors - Yi Fu
human	WB	Adv Sci (Weinh); Transcriptomics-Based Liquid Biopsy for Early Detection of Recurrence in Locally Advanced Gastric Cancer; Authors - Ping'an Ding ; KD Validated
mouse	WB	Aging Cell; Activation of angiotensin-converting enzyme 2/angiotensin (1-7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling; Authors - Ruili Dang
human	IF	Biomaterials; Inhibition of tumor-promoting stroma to enforce subsequently targeting AT1R on tumor cells by pathological inspired micelles.; Authors - Yun Zhu
mouse	WB	Redox Biol; Vitamin D receptor activation regulates microglia polarization and oxidative stress in spontaneously hypertensive rats and angiotensin II-exposed microglial cells: Role of renin-angiotensin system.; Authors - Changmeng Cui
rat,mouse	WB,IF	Free Radic Biol Med; Spinal AT1R contributes to neuroinflammation and neuropathic pain via NOX2-dependent redox signaling in microglia; Authors - Wencui Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kazu (Verified Customer) (12-14-2022)

This antibody worked well on mouse optic nerve sections at optimized dilution of 1:100.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Optic nerve

FH

Wu (Verified Customer) (07-16-2021)

Works good for IHC assay

• Applications: Immunohistochemistry
• Cell Tissue Type: glandular lumen

AGTR1 Antibody Immunohistochemistry validation ( dilution) in glandular lumen (Cat no:25343-1-AP)