AGTR1 Monoklonaler Antikörper

AGTR1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

2C5G11

Rezensionen (1)

Kat-Nr. : 66415-1-Ig

Synonyme

Angiotensin II type-1 receptor, Angiotensin II type 1 receptor, AGTR1B, AGTR1A, AG2S

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  WB analysis of pig heart using 66415-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66415-1-Ig (AGTR1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 66415-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66415-1-Ig (AGTR1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig skeletal muscle using 66415-1-Ig

  pig skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66415-1-Ig (AGTR1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Herzgewebe, Hausschwein-Skelettmuskelgewebe, Rattenherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

66415-1-Ig bindet in WB, ELISA AGTR1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AGTR1 fusion protein Ag14461
• Vollständiger Name: angiotensin II receptor, type 1
• Berechnetes Molekulargewicht: 359 aa, 41 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC022447
• Gene symbol: AGTR1
• Gene ID (NCBI): 185
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Angiotensin II (Ang II), the main effector molecule of the renin-angiotensin system, exerts its actions mainly via interaction with type-1 angiotensin II receptor (AGTR1, also named as AT1R), thereby contributing to blood pressure regulation. AGTR1 mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. By regulating vascular tone, cardiovascular function, salt and water homeostasis, AGTR1 exerts an indispensable physiological role (PMID: 21600887). AGTR1 has been implicated in diverse aspects of human disease, from the regulation of blood pressure and cardiovascular homeostasis to cancer progression (PMID: 26975580).

Publikationen

Species	Application	Title
mouse	WB	J Adv Res; Vitamin D/VDR regulates peripheral energy homeostasis via central renin-angiotensin system.; Authors - Han Su
human	WB	Int J Biol Macromol; A novel angiotensin I-converting enzyme inhibitory peptide APPLRP from Grifola frondosa ameliorated the Ang II-induced vascular modeling in zebrafish model by mediating smooth muscle cells; Authors - Tianyuan Song

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anca (Verified Customer) (10-30-2019)

We could detect AT1R from Plazenta, but only by using Chemiluminiscence. Unfortunately, the pozitive control that we used didn't worked.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Plazenta