AGXT Monoklonaler Antikörper

AGXT Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3E12H1

Kat-Nr. : 67402-1-PBS

Synonyme

3E12H1, AGT, AGT1, AGXT1, Alanine--glyoxylate aminotransferase

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  WB analysis using 67402-1-Ig (same clone as 67402-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67402-1-Ig (AGXT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67402-1-PBS in a different storage buffer formulation.

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  WB analysis of NCI-H1299 using 67402-1-Ig (same clone as 67402-1-PBS)

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67402-1-Ig (AGXT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67402-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 67402-1-Ig (same clone as 67402-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67402-1-Ig (AGXT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67402-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67402-1-Ig (same clone as 67402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67402-1-Ig (AGXT antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67402-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67402-1-Ig (same clone as 67402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67402-1-Ig (AGXT antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67402-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67402-1-PBS bindet in WB, IHC, Indirect ELISA AGXT und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AGXT fusion protein Ag19188
• Vollständiger Name: alanine-glyoxylate aminotransferase
• Berechnetes Molekulargewicht: 392 aa, 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC132819
• Gene symbol: AGXT
• Gene ID (NCBI): 189
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AGXT (AGT) is a key enzyme in glyoxylate detoxification. Mutations of AGXT lead to primary hyperoxaluria type I, a rare metabolic disease which is characterized by deficient AGXT, excessive hepatic oxalate production that leads to renal failure.