AGXT Rekombinanter Antikörper

AGXT Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240305E7

Kat-Nr. : 83385-6-PBS

Synonyme

EC:2.6.1.44, Alanine--glyoxylate aminotransferase, AGXT1, AGT1, AGT

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  WB analysis using 83385-6-RR (same clone as 83385-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83385-6-RR (AGXT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83385-6-PBS in a different storage buffer formulation.

• 

  WB analysis using 83385-6-RR (same clone as 83385-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83385-6-RR (AGXT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83385-6-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83385-6-RR (same clone as 83385-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AGXT antibody (83385-6-RR, Clone: 240305E7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83385-6-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 83385-6-RR (same clone as 83385-6-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug AGXT Recombinant antibody (83385-6-RR, Clone:240305E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83385-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83385-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83385-6-RR against Human AGXT were performed. The affinity constant is 0.226 nM.

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Geprüfte Anwendungen

Produktinformation

83385-6-PBS bindet in WB, IF/ICC, FC (Intra), ELISA AGXT und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: AGXT fusion protein Ag18062
• Vollständiger Name: alanine-glyoxylate aminotransferase
• Berechnetes Molekulargewicht: 392 aa, 43 kDa
• GenBank-Zugangsnummer: BC132819
• Gene symbol: AGXT
• Gene ID (NCBI): 189
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.