AHCY Polyklonaler Antikörper

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• KD/KO Validated

AHCY Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

24

Rezensionen (1)

Kat-Nr. : 10757-2-AP

Synonyme

Adenosylhomocysteinase, AdoHcyase, AHCY, S adenosylhomocysteine hydrolase, SAHH

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Galerie der Validierungsdaten

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  WB analysis using 10757-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10757-2-AP (AHCY antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10757-2-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10757-2-AP (AHCY antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10757-2-AP

  WB result of AHCY antibody (10757-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AHCY transfected HeLa cells.

• 

  WB analysis of HepG2 using 10757-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10757-2-AP (AHCY Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 10757-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10757-2-AP (AHCY antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 20 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

10757-2-AP bindet in WB, IP, IHC, IF, ELISA AHCY und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AHCY fusion protein Ag1208
• Vollständiger Name: S-adenosylhomocysteine hydrolase
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 45-48 kDa
• GenBank-Zugangsnummer: BC011606
• Gene symbol: AHCY
• Gene ID (NCBI): 191
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).

Publikationen

Species	Application	Title
human	WB	Cell Stem Cell; Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.; Authors - Rashim Pal Singh
human	WB, IP	Nat Commun; H19 lncRNA alters DNA methylation genome wide by regulating S-adenosylhomocysteine hydrolase.; Authors - Jichun Zhou ; KD Validated
human	WB	Autophagy; AHCYL1 senses SAH to inhibit autophagy through interaction with PIK3C3 in an MTORC1-independent manner.; Authors - Wei Huang ; KD Validated
human	WB	Oncogene; PMID: 27775072; Authors - T Zhong
human	WB,IHC	Clin Cancer Res; Digital transcript profile analysis with aRNA-LongSAGE validates FERMT1 as a potential novel prognostic marker for colon cancer.; Authors - Fan Junwei J
mouse	WB	Arterioscler Thromb Vasc Biol; Increased plasma S-adenosylhomocysteine-accelerated atherosclerosis is associated with epigenetic regulation of endoplasmic reticulum stress in apoE-/- mice.; Authors - Yunjun Xiao ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kamal (Verified Customer) (01-26-2024)

Antibody worked well for western blot analysis when diluted with 1X TBST at the provided dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Mouse liver tissue