AHCY Monoklonaler Antikörper

AHCY Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1F9B10

Kat-Nr. : 66019-1-Ig

Synonyme

Adenosylhomocysteinase, AdoHcyase, AHCY, S adenosylhomocysteine hydrolase, SAHH

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 66019-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66019-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 66019-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 66019-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66019-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 66019-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 66019-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 66019-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 66019-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 66019-1-Ig

  Immunofluorescent analysis of Hela cells, using AHCY antibody 66019-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hodengewebe, L02-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66019-1-Ig bindet in WB, IHC, IF, ELISA AHCY und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AHCY fusion protein Ag18036
• Vollständiger Name: S-adenosylhomocysteine hydrolase
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC011606
• Gene symbol: AHCY
• Gene ID (NCBI): 191
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).

Publikationen

Species	Application	Title
human	WB	Cell Death Differ; Quantitative reactive cysteinome profiling reveals a functional link between ferroptosis and proteasome-mediated degradation; Authors - Yankun Wang