AHCY Monoklonaler Antikörper

AHCY Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1F9B10

Kat-Nr. : 66019-1-Ig

Synonyme

Adenosylhomocysteinase, AdoHcyase, AHCY, S adenosylhomocysteine hydrolase, SAHH

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  WB analysis using 66019-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 66019-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66019-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66019-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 66019-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 66019-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66019-1-Ig (AHCY antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66019-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66019-1-Ig (AHCY antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 66019-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 66019-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 66019-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 66019-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 66019-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AHCY antibody (66019-1-Ig, Clone: 1F9B10 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Hodengewebe, L02-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Plazenta-Gewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66019-1-Ig bindet in WB, IHC, IF/ICC, ELISA AHCY und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AHCY fusion protein Ag18036
• Vollständiger Name: S-adenosylhomocysteine hydrolase
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC011606
• Gene symbol: AHCY
• Gene ID (NCBI): 191
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).

Publikationen

Species	Application	Title
human	WB	Cell Death Differ; Quantitative reactive cysteinome profiling reveals a functional link between ferroptosis and proteasome-mediated degradation; Authors - Yankun Wang