AHCYL1 Polyklonaler Antikörper

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• KD/KO Validated

AHCYL1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

20

Kat-Nr. : 10658-3-AP

Synonyme

AdoHcyase 2, AHCYL1, DCAL, IRBIT, PRO0233, XPVKONA

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 10658-3-AP

  WB result of AHCYL1 antibody (10658-3-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AHCYL1 transfected HeLa cells.

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  WB analysis of Jurkat using 10658-3-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10658-3-AP (AHCYL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 10658-3-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10658-3-AP (AHCYL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10658-3-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10658-3-AP (AHCYL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10658-3-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10658-3-AP (AHCYL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10658-3-AP

  IP Result of anti-AHCYL1 (IP:10658-3-AP, 4ug; Detection:10658-3-AP 1:1000) with HeLa cells lysate 2400ug.

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  IHC staining of human liver cancer using 10658-3-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10658-3-AP (AHCYL1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10658-3-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10658-3-AP (AHCYL1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10658-3-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AHCYL1 antibody (10658-3-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, Maus-Thymusgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 19 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Produktinformation

10658-3-AP bindet in WB, IP, IHC, IF, ELISA AHCYL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AHCYL1 fusion protein Ag1017
• Vollständiger Name: S-adenosylhomocysteine hydrolase-like 1
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC007576
• Gene symbol: AHCYL1
• Gene ID (NCBI): 10768
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S-adenosylhomocysteine hydrolase-like protein 1 (AHCYL1), also known as IP3 receptor-binding protein released with IP3 (IRBIT), regulates IP3-induced Ca2+ release into the cytoplasm of cells(PMID:23145124). This protein is also named as DCAL, XPVKONA and belongs to the adenosylhomocysteinase family. AHCYL1 mRNA and protein are most abundant in the glandular epithelium of adenocarcinoma of cancerous, but not normal and AHCYL1 protein is predominantly in the nucleus(PMID:22826361). It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
pig	WB	J Agric Food Chem; Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets.; Authors - Yimin Jia
rat	WB	J Agric Food Chem; Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.; Authors - Shu Yang
rat	WB	Mol Nutr Food Res; Transgenerational Inheritance of Betaine-Induced Epigenetic Alterations in Estrogen-Responsive IGF-2/IGFBP2 Genes in Rat Hippocampus.; Authors - Yang Yang
rat	WB	Mol Nutr Food Res; Maternal betaine supplementation in rats induces intergenerational changes in hepatic IGF-1 expression and DNA methylation.; Authors - Nannan Zhao
human	WB	Pharmaceutics; Protective Role of IRBIT on Sodium Bicarbonate Cotransporter-n1 for Migratory Cancer Cells.; Authors - Soyoung Hwang
chicken	WB	J Nutr; Corticosterone-Induced Lipogenesis Activation and Lipophagy Inhibition in Chicken Liver Are Alleviated by Maternal Betaine Supplementation.; Authors - Yun Hu