AHR Monoklonaler Antikörper

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• KD/KO Validated

AHR Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(56)

CloneNo.

2D1F9

Rezensionen (1)

Kat-Nr. : 67785-1-Ig

Synonyme

Class E basic helix-loop-helix protein 76, bHLHe76, Ah receptor, 2D1F9

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  WB analysis using 67785-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67785-1-Ig (AHR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67785-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67785-1-Ig (AHR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67785-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67785-1-Ig (AHR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67785-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67785-1-Ig (AHR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67785-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67785-1-Ig (AHR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 67785-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67785-1-Ig (AHR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67785-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67785-1-Ig (AHR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67785-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67785-1-Ig (AHR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human breast cancer using 67785-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using AHR antibody (67785-1-Ig, Clone: 2D1F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Blue (DAPI).

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  IF Staining of mouse testis using 67785-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AHR antibody (67785-1-Ig, Clone: 2D1F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 67785-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AHR antibody (67785-1-Ig, Clone: 2D1F9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, 4T1-Zellen, Caco-2-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, LNCaP-Zellen, MCF-7-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Mammakarzinomgewebe, Maushodengewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:200-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 48 publications below
• IHC: See 6 publications below
• IF: See 10 publications below
• FC: See 1 publications below

Produktinformation

67785-1-Ig bindet in WB, IHC, IF/ICC, IF-P, ELISA AHR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AHR fusion protein Ag28935
• Vollständiger Name: aryl hydrocarbon receptor
• Berechnetes Molekulargewicht: 848 aa, 96 kDa
• Beobachtetes Molekulargewicht: 105-110 kDa
• GenBank-Zugangsnummer: BC070080
• Gene symbol: AHR
• Gene ID (NCBI): 196
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

Publikationen

Species	Application	Title
human	WB,IF	Phytother Res; Quercetin ameliorates ulcerative colitis by activating aryl hydrocarbon receptor to improve intestinal barrier integrity; Authors - Xiaojing Wang
mouse	WB	NPJ Regen Med; Aryl hydrocarbon receptor regulates IL-22 receptor expression on thymic epithelial cell and accelerates thymus regeneration; Authors - Jingyi Shen
		Cell Rep; Hypoxia-induced PRMT1 methylates HIF2β to promote breast tumorigenesis via enhancing glycolytic gene transcription; Authors - Wen-Juan Li
mouse	IF	Ecotoxicol Environ Saf; Hesperetin protects hippocampal neurons from the neurotoxicity of Aflatoxin B1 in mice; Authors - Chao Song
rat	WB	Biomater Sci; Glioma-targeted multifunctional nanoparticles to co-deliver camptothecin and curcumin for enhanced chemo-immunotherapy.; Authors - Zian Wang
mouse	WB,IF	iScience; StemRegenin 1 attenuates the RANKL-induced osteoclastogenesis via inhibiting AhR-c-src-NF-κB/p-ERK MAPK-NFATc1 signaling pathway; Authors - Shengji Zhou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Bartosz (Verified Customer) (02-09-2023)

Perfect Abs, the bands are clear and very strong, even in case of small of amount of protein, loaded into gel (20 ug). Just perfect, highly recommend.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: SH-SY5Y cells; A549 cells; CACO-2 cells; BJ cells