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AHR Monoklonaler Antikörper
AHR Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA
Wirt / Isotyp
Maus / IgG2b
Getestete Reaktivität
human, Maus, Ratte und mehr (2)
Anwendung
WB, IHC, IF/ICC, IF-P, ELISA
Konjugation
Unkonjugiert
CloneNo.
2D1F9
Kat-Nr. : 67785-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | A549-Zellen, 4T1-Zellen, Caco-2-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, LNCaP-Zellen, MCF-7-Zellen, PC-3-Zellen |
| Erfolgreiche Detektion in IHC | humanes Mammakarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF-P | humanes Mammakarzinomgewebe, Maushodengewebe |
| Erfolgreiche Detektion in IF/ICC | HepG2-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunhistochemie (IHC) | IHC : 1:200-1:500 |
| Immunfluoreszenz (IF)-P | IF-P : 1:200-1:800 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 3 publications below |
| WB | See 48 publications below |
| IHC | See 6 publications below |
| IF | See 10 publications below |
| FC | See 1 publications below |
Produktinformation
67785-1-Ig bindet in WB, IHC, IF/ICC, IF-P, ELISA AHR und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Hausschwein, Maus, Ratte, Zebrafisch |
| Wirt / Isotyp | Maus / IgG2b |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | AHR fusion protein Ag28935 |
| Vollständiger Name | aryl hydrocarbon receptor |
| Berechnetes Molekulargewicht | 848 aa, 96 kDa |
| Beobachtetes Molekulargewicht | 105-110 kDa |
| GenBank-Zugangsnummer | BC070080 |
| Gene symbol | AHR |
| Gene ID (NCBI) | 196 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for AHR antibody 67785-1-Ig | Protokoll herunterladen |
| IHC protocol for AHR antibody 67785-1-Ig | Protokoll herunterladenl |
| IF protocol for AHR antibody 67785-1-Ig | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Phytother Res Quercetin ameliorates ulcerative colitis by activating aryl hydrocarbon receptor to improve intestinal barrier integrity | ||
NPJ Regen Med Aryl hydrocarbon receptor regulates IL-22 receptor expression on thymic epithelial cell and accelerates thymus regeneration | ||
Cell Rep Hypoxia-induced PRMT1 methylates HIF2β to promote breast tumorigenesis via enhancing glycolytic gene transcription | ||
Ecotoxicol Environ Saf Hesperetin protects hippocampal neurons from the neurotoxicity of Aflatoxin B1 in mice | ||
Biomater Sci Glioma-targeted multifunctional nanoparticles to co-deliver camptothecin and curcumin for enhanced chemo-immunotherapy. | ||
iScience StemRegenin 1 attenuates the RANKL-induced osteoclastogenesis via inhibiting AhR-c-src-NF-κB/p-ERK MAPK-NFATc1 signaling pathway |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Bartosz (Verified Customer) (02-09-2023) | Perfect Abs, the bands are clear and very strong, even in case of small of amount of protein, loaded into gel (20 ug). Just perfect, highly recommend.
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