AIDA Monoklonaler Antikörper

AIDA Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D11A1

Kat-Nr. : 68153-1-Ig

Synonyme

AIDA, C1orf80, FLJ12806, FLJ32421, RP11 378J18.7

---

Galerie der Validierungsdaten

• 

  WB analysis using 68153-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68153-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 68153-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A431 using 68153-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using AIDA antibody (68153-1-Ig, Clone: 1D11A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, HepG2-Zellen, HT-29-Zellen, K-562-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68153-1-Ig bindet in WB, IF, ELISA AIDA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AIDA fusion protein Ag20597
• Vollständiger Name: axin interactor, dorsalization associated
• Berechnetes Molekulargewicht: 306 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC015535
• Gene symbol: AIDA
• Gene ID (NCBI): 64853
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AIDA, also named as C1orf80, acts as a ventralizing factor during embryogenesis. It inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. It's highest expression in the heart and skeletal muscle. AIDA has three isoforms with MW 35 kDa, 16 kDa and 26 kDa.