AIDA Monoklonaler Antikörper

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AIDA Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D11A1

Kat-Nr. : 68153-1-PBS

Synonyme

1D11A1, Axin interaction partner and dorsalization antagonist, Axin interactor, dorsalization-associated protein, C1orf80

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  WB analysis using 68153-1-Ig (same clone as 68153-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68153-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68153-1-Ig (same clone as 68153-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68153-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68153-1-Ig (same clone as 68153-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68153-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68153-1-Ig (same clone as 68153-1-PBS)

  WB result of AIDA antibody (68153-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AIDA transfected HeLa cells. This data was developed using the same antibody clone with 68153-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68153-1-Ig (same clone as 68153-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using AIDA antibody (68153-1-Ig, Clone: 1D11A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68153-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68153-1-PBS bindet in WB, IF/ICC, Indirect ELISA AIDA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AIDA fusion protein Ag20597
• Vollständiger Name: axin interactor, dorsalization associated
• Berechnetes Molekulargewicht: 306 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC015535
• Gene symbol: AIDA
• Gene ID (NCBI): 64853
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AIDA, also named as C1orf80, acts as a ventralizing factor during embryogenesis. It inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. It's highest expression in the heart and skeletal muscle. AIDA has three isoforms with MW 35 kDa, 16 kDa and 26 kDa.