AIF Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

AIF Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(89)

Rezensionen (2)

Kat-Nr. : 17984-1-AP

Synonyme

AIFM1, PDCD8, EC:1.6.99.-, Apoptosis-inducing factor 1, mitochondrial

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  WB analysis of HeLa using 17984-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17984-1-AP (AIF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17984-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17984-1-AP (AIF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17984-1-AP

  WB result of AIF antibody (17984-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AIF transfected HeLa cells.

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  IP experiment of HeLa using 17984-1-AP

  IP result of anti-AIF (IP:17984-1-AP, 3ug; Detection:17984-1-AP 1:2000) with HeLa cells lysate 1320ug.

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  IHC staining of human kidney using 17984-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17984-1-AP (AIF Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 17984-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17984-1-AP (AIF Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse kidney using 17984-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17984-1-AP (AIF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 17984-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17984-1-AP (AIF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 17984-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17984-1-AP (AIF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 17984-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AIF antibody (17984-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Mausnierengewebe, Maus-Magengewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 78 publications below
• IHC: See 7 publications below
• IF: See 21 publications below

Produktinformation

17984-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA AIF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AIF fusion protein Ag12400
• Vollständiger Name: apoptosis-inducing factor, mitochondrion-associated, 1
• Berechnetes Molekulargewicht: 609 aa, 66 kDa
• Beobachtetes Molekulargewicht: 67 kDa, 57 kDa
• GenBank-Zugangsnummer: BC111065
• Gene symbol: AIF
• Gene ID (NCBI): 9131
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID: 25101006).

Publikationen

Species	Application	Title
mouse,human	WB	Cell Rep; Newly synthesized AIFM1 determines the hypersensitivity of T lymphocytes to STING activation-induced cell apoptosis; Authors - Wangsheng Ji ; KD Validated
rat	WB	Phytomedicine; LC-MS based metabonomics study on protective mechanism of ESWW in cerebral ischemia via CYTC/Apaf-1/NDRG4 pathway; Authors - Yan Liang
rat	WB	Oxid Med Cell Longev; miRNA-27a Transcription Activated by c-Fos Regulates Myocardial Ischemia-Reperfusion Injury by Targeting ATAD3a.; Authors - Yandong Bao
human	WB	Elife; Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.; Authors - Maxence Le Vasseur
human	WB	PLoS Pathog; GP73 represses host innate immune response to promote virus replication by facilitating MAVS and TRAF6 degradation.; Authors - Xuewu Zhang
human	WB	J Med Chem; Anticancer Effects of Honokiol via Mitochondrial Dysfunction Are Strongly Enhanced by the Mitochondria-Targeting Carrier Berberine.; Authors - Xiaojia Shi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tom (Verified Customer) (11-13-2020)

HeLa cells permeablisied (0.1% Triton X-100 in PBS) then blocked (1% BSA in PBS). Cells incubated with primary antibody (1:100) for 1 hour, 3xPBS prior to 1 hour incubation with goat anti-rabbit 488 Alexa Fluor secondary antibody.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HeLa

AIF Antibody Immunofluorescence validation (1:100 dilution) in HeLa (Cat no:17984-1-AP)

FH

Praveen (Verified Customer) (05-17-2019)

Excellent Antibody

• Applications: Western Blot,
• Cell Tissue Type: AC16

AIF Antibody Western Blot, validation ( dilution) in AC16 (Cat no:17984-1-AP)