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AIF Rekombinanter Antikörper

AIF Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250772F8

Kat-Nr. : 86156-3-PBS

Synonyme

AIFM1, Apoptosis-inducing factor 1, mitochondrial, EC:1.6.99.-, PDCD8

Filter:
  • Western Blot (WB) analysis of various lysates using AIF Recombinant monoclonal antibody (86156-3-RR)

    WB analysis using 86156-3-RR (same clone as 86156-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86156-3-RR (AIF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86156-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86156-3-RR

    Affinity and Kinetic Characterization of 86156-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86156-3-RR against Human AIF were performed. The affinity constant is 89.1 pM.

Geprüfte Anwendungen

Produktinformation

86156-3-PBS bindet in WB, Indirect ELISA AIF und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen AIF fusion protein Ag13268
Vollständiger Name apoptosis-inducing factor, mitochondrion-associated, 1
Berechnetes Molekulargewicht 609 aa, 66 kDa
Beobachtetes Molekulargewicht67 kDa
GenBank-ZugangsnummerBC111065
Gene symbol AIF
Gene ID (NCBI) 9131
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).