AIFM2/ FSP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

AIFM2/ FSP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(170)

Rezensionen (1)

Kat-Nr. : 20886-1-AP

Synonyme

AMID, AIFM2, FSP1, EC:1.6.5.-, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death

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  WB analysis using 20886-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20886-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20886-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 20886-1-AP

  IP result of anti-AIFM2/ FSP1 (IP:20886-1-AP, 4ug; Detection:20886-1-AP 1:3000) with L02 cells lysate 1040 ug.

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  IHC staining of mouse liver using 20886-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 20886-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 20886-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using AIFM2/ FSP1 antibody (20886-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HeLa-Zellen, HepG2-Zellen, Rattenlebergewebe
• Erfolgreiche IP: L02-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 150 publications below
• IHC: See 19 publications below
• IF: See 24 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below

Produktinformation

20886-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA AIFM2/ FSP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ente, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AIFM2/ FSP1 fusion protein Ag13412
• Vollständiger Name: apoptosis-inducing factor, mitochondrion-associated, 2
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC023601
• Gene symbol: AIFM2/ FSP1
• Gene ID (NCBI): 84883
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis-associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis-Inducing Factor), a mitochondrion-associated enzyme that relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.

Publikationen

Species	Application	Title
human	WB	Cell; ALDH7A1 protects against ferroptosis by generating membrane NADH and regulating FSP1; Authors - Jia-Shu Yang
human	WB	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
human	WB	Nature; The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis.; Authors - Kirill Bersuker
human	WB,IHC	Drug Resist Updat; Upregulation of CoQ shifts ferroptosis dependence from GPX4 to FSP1 in acquired radioresistance; Authors - Xu Lin
human	WB	Protein Cell; Proteomic analysis of ferroptosis pathways reveals a role of CEPT1 in suppressing ferroptosis; Authors - Xiaoguang Liu
mouse	WB	Nat Commun; Targeting ferroptosis by poly(acrylic) acid coated Mn3O4 nanoparticles alleviates acute liver injury; Authors - Xinyi Shan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Selin (Verified Customer) (03-14-2024)

We've been having problems to identify this protein, but luckily this antibody worked perfectly.

• Applications: Western Blot