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AIFM2/ FSP1 Polyklonaler Antikörper
AIFM2/ FSP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (2)
Anwendung
WB, IHC, IF/ICC, IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 20886-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Mausnierengewebe, HeLa-Zellen, HepG2-Zellen, Rattenlebergewebe | 
| Erfolgreiche IP | L02-Zellen | 
| Erfolgreiche Detektion in IHC | Mauslebergewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. | 
| Erfolgreiche Detektion in IF/ICC | A549-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 | 
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunhistochemie (IHC) | IHC : 1:50-1:500 | 
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 7 publications below | 
| WB | See 150 publications below | 
| IHC | See 19 publications below | 
| IF | See 24 publications below | 
| IP | See 4 publications below | 
| CoIP | See 2 publications below | 
Produktinformation
20886-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA AIFM2/ FSP1 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte | 
| In Publikationen genannte Reaktivität | human, Ente, Hausschwein, Maus, Ratte | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Polyklonal | 
| Typ | Antikörper | 
| Immunogen | AIFM2/ FSP1 fusion protein Ag13412 | 
| Vollständiger Name | apoptosis-inducing factor, mitochondrion-associated, 2 | 
| Berechnetes Molekulargewicht | 41 kDa | 
| Beobachtetes Molekulargewicht | 41 kDa | 
| GenBank-Zugangsnummer | BC023601 | 
| Gene symbol | AIFM2/ FSP1 | 
| Gene ID (NCBI) | 84883 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Antigen-Affinitätsreinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis-associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis-Inducing Factor), a mitochondrion-associated enzyme that relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for AIFM2/ FSP1 antibody 20886-1-AP | Protokoll herunterladen | 
| IHC protocol for AIFM2/ FSP1 antibody 20886-1-AP | Protokoll herunterladenl | 
| IF protocol for AIFM2/ FSP1 antibody 20886-1-AP | Protokoll herunterladen | 
| IP protocol for AIFM2/ FSP1 antibody 20886-1-AP | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Cell ALDH7A1 protects against ferroptosis by generating membrane NADH and regulating FSP1 | ||
| Cell Res Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression | ||
| Drug Resist Updat Upregulation of CoQ shifts ferroptosis dependence from GPX4 to FSP1 in acquired radioresistance | ||
| Protein Cell Proteomic analysis of ferroptosis pathways reveals a role of CEPT1 in suppressing ferroptosis | ||
| Nat Commun Targeting ferroptosis by poly(acrylic) acid coated Mn3O4 nanoparticles alleviates acute liver injury | 
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Selin (Verified Customer) (03-14-2024) | We've been having problems to identify this protein, but luckily this antibody worked perfectly. 
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