AIFM2/ FSP1 Polyklonaler Antikörper

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• KD/KO Validated

AIFM2/ FSP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20886-1-PBS

Synonyme

AMID, AIFM2, FSP1, EC:1.6.5.-, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death

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  WB analysis using 20886-1-AP (same clone as 20886-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20886-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20886-1-AP (same clone as 20886-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20886-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20886-1-AP (same clone as 20886-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20886-1-PBS in a different storage buffer formulation.

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  IP experiment of L02 using 20886-1-AP (same clone as 20886-1-PBS)

  IP result of anti-AIFM2/ FSP1 (IP:20886-1-AP, 4ug; Detection:20886-1-AP 1:3000) with L02 cells lysate 1040 ug. This data was developed using the same antibody clone with 20886-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 20886-1-AP (same clone as 20886-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20886-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 20886-1-AP (same clone as 20886-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20886-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 20886-1-AP (same clone as 20886-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using AIFM2/ FSP1 antibody (20886-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 20886-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20886-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA AIFM2/ FSP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AIFM2/ FSP1 fusion protein Ag13412
• Vollständiger Name: apoptosis-inducing factor, mitochondrion-associated, 2
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC023601
• Gene symbol: AIFM2/ FSP1
• Gene ID (NCBI): 84883
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis-associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis-Inducing Factor), a mitochondrion-associated enzyme that relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.