AIG1 Polyklonaler Antikörper

AIG1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 14468-1-AP

Synonyme

AIG 1, AIG1, androgen induced 1

---

• 

  WB analysis using 14468-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 14468-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse heart using 14468-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse testis using 14468-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14468-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of rat heart using 14468-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat ovary using 14468-1-AP

  rat ovary tissue were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 14468-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14468-1-AP (AIG1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary tumor using 14468-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14468-1-AP (AIG1 antibody) at dilution of 1:100 (under 10x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Mausherzgewebe, Maus-Eierstockgewebe, Maushodengewebe, PC-3-Zellen, Rattenherzgewebe, Ratten-Eierstockgewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

14468-1-AP bindet in WB, IHC, ELISA AIG1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AIG1 fusion protein Ag5836
• Vollständiger Name: androgen-induced 1
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28-32 kDa
• GenBank-Zugangsnummer: BC025278
• Gene symbol: AIG1
• Gene ID (NCBI): 51390
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
	WB	Nat Commun; Inflammation causes insulin resistance in mice via interferon regulatory factor 3 (IRF3)-mediated reduction in FAHFA levels; Authors - Shuai Yan