AIM2 Polyklonaler Antikörper

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• KD/KO Validated

AIM2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(34)

Kat-Nr. : 20590-1-AP

Synonyme

Interferon-inducible protein AIM2, PYHIN4

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  WB analysis using 20590-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20590-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 20590-1-AP

  IP result of anti-AIM2 (IP:20590-1-AP, 4ug; Detection:20590-1-AP 1:600) with Raji cells lysate 2200 ug.

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  IHC staining of human testis using 20590-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20590-1-AP (AIM2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 20590-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20590-1-AP (AIM2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 20590-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using AIM2 antibody (20590-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen, Raji-Zellen
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 29 publications below
• IHC: See 11 publications below

Produktinformation

20590-1-AP bindet in WB, IHC, IP, ELISA AIM2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AIM2 fusion protein Ag14520
• Vollständiger Name: absent in melanoma 2
• Berechnetes Molekulargewicht: 343 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39-42 kDa
• GenBank-Zugangsnummer: BC010940
• Gene symbol: AIM2
• Gene ID (NCBI): 9447
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria (PMID:22695634). Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity (PMID:16432157,20566831). It has been reported that AIM2 protein levels are significantly elevated in alveolar macrophages of patients with IPF and bleomycin-treated mice (PMID: 38078661). AIM2 inflammasome was also significantly activated, which was also confirmed in Aβ-treated microglial cells (PMID: 34861878)

Publikationen

Species	Application	Title
mouse	WB	Cell Mol Immunol; NLRP3-dependent pyroptosis is required for HIV-1 gp120-induced neuropathology.; Authors - Xiaolong He ; KD Validated
mouse	WB	Mucosal Immunol; AIM2 contributes to the maintenance of intestinal integrity via Akt and protects against Salmonella mucosal infection.; Authors - G-Q Hu
human	WB	J Agric Food Chem; Integrative Gut Microbiota and Metabolomic Analyses Reveal the PANoptosis- and Ferroptosis-Related Mechanisms of Chrysoeriol in Inhibiting Melanoma; Authors - Yuxi Liu
human	IHC	Front Immunol; Cytokine Secretion and Pyroptosis of Thyroid Follicular Cells Mediated by Enhanced NLRP3, NLRP1, NLRC4, and AIM2 Inflammasomes Are Associated With Autoimmune Thyroiditis.; Authors - Qingling Guo
human	WB	Front Cell Dev Biol; Autophagy-Based Unconventional Secretory for AIM2 Inflammasome Drives DNA Damage Resistance During Intervertebral Disc Degeneration.; Authors - Shuai Li ; KD Validated
human	WB,IHC	Front Immunol; Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma; Authors - Liangmin Fu