AIM2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

AIM2 Monoklonal Antikörper für WB, IF/ICC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Kaninchen, Maus, Ratte

Anwendung

WB, IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3E12A8

Kat-Nr. : CL488-66902

Synonyme

3E12A8, Interferon-inducible protein AIM2, PYHIN4

---

• 

  WB analysis using CL488-66902

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66902 (AIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using CL488-66902

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 AIM2 antibody (CL488-66902, Clone: 3E12A8 ) at dilution of 1:200.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66902 bindet in WB, IF/ICC AIM2 und zeigt Reaktivität mit human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AIM2 fusion protein Ag14607
• Vollständiger Name: absent in melanoma 2
• Berechnetes Molekulargewicht: 343 aa, 39 kDa
• Beobachtetes Molekulargewicht: 37-39 and 53 kDa
• GenBank-Zugangsnummer: BC010940
• Gene symbol: AIM2
• Gene ID (NCBI): 9447
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID: 22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]