AK2 Monoklonaler Antikörper

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• KD/KO Validated

AK2 Monoklonal Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3H2C10

Kat-Nr. : 66127-1-PBS

Synonyme

3H2C10, adenylate kinase 2, Adenylate kinase 2, mitochondrial, N-terminally processed, Adenylate monophosphate kinase, ADK2

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  WB analysis using 66127-1-Ig (same clone as 66127-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66127-1-Ig (AK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66127-1-Ig (same clone as 66127-1-PBS)

  WB result of AK2 antibody (66127-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AK2 transfected HeLa cells. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66127-1-Ig (same clone as 66127-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66127-1-Ig (AK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AK2 antibody (66127-1-Ig, Clone: 3H2C10 ) at dilution of 1:5024 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 66127-1-Ig (AK2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunofluorescent analysis of HeLa cells using 66127-1-Ig (AK2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66127-1-Ig (same clone as 66127-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug AK2 Monoclonal antibody (66127-1-Ig, Clone:3H2C10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50407-1

  Cytometric bead array standard curve of MP50407-1, AK2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66127-1-PBS. Detection antibody: 66127-2-PBS. Standard:Ag17911. Range: 0.195-50 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66127-1-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA AK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AK2 fusion protein Ag17911
• Vollständiger Name: adenylate kinase 2
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC009405
• Gene symbol: AK2
• Gene ID (NCBI): 204
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AK2(Adenylate kinase 2, mitochondrial ) is also named as ADK2 and belongs to the adenylate kinase family. It plays a unique role in energy metabolism and energy transfer by regulating the ATP/ADP rate between the cytoplasma matrix and the mitochondria and it is increased expression during adipocyte and B cell differentiation(PMID:23020757). AK2 catalyzes the reversible transfer of a phosphoryl group from ATP to AMP(PMID:19043417). Defects in AK2 are the cause of reticular dysgenesis (RDYS)(PMID:19043416). It has 6 isoforms produced by alternative splicing.