AK2 Rekombinanter Antikörper

AK2 Rekombinant Antikörper für WB, IHC, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242899H7

Kat-Nr. : 85406-3-PBS

Synonyme

242899H7, adenylate kinase 2, Adenylate kinase 2, mitochondrial, N-terminally processed, Adenylate monophosphate kinase, ADK2

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  WB analysis using 85406-3-RR (same clone as 85406-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85406-3-RR (AK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85406-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP01927-3

  The mean AK2 concentration was determined to be 6.31 ng/mL in HeLa cell extract based on a 2.10 mg/mL extract load and 5.60 ng/mL in HL-60 cell extract based on a 1.50 mg/mL extract load.

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  Sandwich ELISA standard curve of MP01927-3

  Sandwich ELISA standard curve of MP01927-3, Human AK2 Recombinant Matched Antibody Pair - PBS only. 85406-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1464. 85406-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  IHC staining of mouse kidney using 85406-3-RR (same clone as 85406-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85406-3-RR (AK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85406-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 85406-3-RR (same clone as 85406-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85406-3-RR (AK2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85406-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85406-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85406-3-RR against Human AK2 were performed. The affinity constant is 0.428 nM.

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Geprüfte Anwendungen

Produktinformation

85406-3-PBS bindet in WB, IHC, Sandwich ELISA, Indirect ELISA AK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: AK2 fusion protein Ag1464
• Vollständiger Name: adenylate kinase 2
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC009405
• Gene symbol: AK2
• Gene ID (NCBI): 204
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AK2(Adenylate kinase 2, mitochondrial ) is also named as ADK2 and belongs to the adenylate kinase family. It plays a unique role in energy metabolism and energy transfer by regulating the ATP/ADP rate between the cytoplasma matrix and the mitochondria and it is increased expression during adipocyte and B cell differentiation(PMID:23020757). AK2 catalyzes the reversible transfer of a phosphoryl group from ATP to AMP(PMID:19043417). Defects in AK2 are the cause of reticular dysgenesis (RDYS)(PMID:19043416). It has 6 isoforms produced by alternative splicing.