AK3 Polyklonaler Antikörper

AK3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12562-1-PBS

Synonyme

Adenylate kinase 3 alpha-like 1, AK 3, AK3L1, AK6, AKL3L

---

• 

  WB analysis using 12562-1-AP (same clone as 12562-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Y79 using 12562-1-AP (same clone as 12562-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse kidney using 12562-1-AP (same clone as 12562-1-PBS)

  IP result of anti-AK3 (IP:12562-1-AP, 3ug; Detection:12562-1-AP 1:500) with mouse kidney tissue lysate 4400ug. This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 12562-1-AP (same clone as 12562-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12562-1-AP (AK3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 12562-1-AP (same clone as 12562-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12562-1-AP (AK3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 12562-1-AP (same clone as 12562-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12562-1-AP (AK3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12562-1-AP (same clone as 12562-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AK3 antibody (12562-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

12562-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA AK3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AK3 fusion protein Ag3146
• Vollständiger Name: adenylate kinase 3
• Berechnetes Molekulargewicht: 227 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC013771
• Gene symbol: AK3
• Gene ID (NCBI): 50808
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AK3(Adenylate kinase 3) is also named as AK3L1, AK6, AKL3L and belongs to the adenylate kinase family. It is located in the mitochondrial matrix and is a GTP:AMP phosphotransferase(PMID:2546792). AK3 can be detected AK3 at an apparent molecular mass of 28 kD by western blot analysis and subcellular fractionation of mouse liver and kidney detected Ak3 in the mitochondrial fraction(PMID:11485571). This antibody may also recognize AK4 due to the high homology.