AK3L1 Polyklonaler Antikörper

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• KD/KO Validated

AK3L1 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 13206-1-AP

Synonyme

Adenylate kinase 3 like, adenylate kinase 3 like 1, AK3, AK3L1, AK4, ATP AMP transphosphorylase

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 13206-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13206-1-AP (AK3L1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13206-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13206-1-AP (AK3L1 antibody) at dilution of 1:1800 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 13206-1-AP

  IP result of anti-AK3L1 (IP:13206-1-AP, 4ug; Detection:13206-1-AP 1:300) with MCF-7 cells lysate 3200 ug.

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  IHC staining of human breast cancer using 13206-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13206-1-AP (AK3L1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13206-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13206-1-AP (AK3L1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 13206-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 13206-1-AP (AK3L1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, humanes Lebergewebe
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3600
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

13206-1-AP bindet in WB, IP, IHC, IF, ELISA AK3L1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AK3L1 fusion protein Ag3966
• Vollständiger Name: adenylate kinase 3-like 1
• Berechnetes Molekulargewicht: 223 aa, 25 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC040224
• Gene symbol: AK3L1
• Gene ID (NCBI): 205
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AK3L1(Adenylate kinase 3-like) is also named as AK4, AK3L1 and belongs to the adenylate kinase family. It catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It is important for maintenance of homeostasis of the adenine and guanine nucleotide pools. Expression is highest in kidney and heart, moderate in liver, weak in brain, and barely detectable in placenta and lung by northern blot(PMID:11485571). This antibody may also recognize AK3 due to the high homology.

Publikationen

Species	Application	Title
human	WB	Cancer Cell Int; The miR-199a-3p regulates the radioresistance of esophageal cancer cells via targeting the AK4 gene.; Authors - Chunbao Zang ; KD Validated
rat	WB,IHC	Exp Brain Res; MicroRNA-124 conducts neuroprotective effect via inhibiting AK4/ATF3 after subarachnoid hemorrhage; Authors - Wei Jiang