AKAP1 Polyklonaler Antikörper

AKAP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15618-1-PBS

Synonyme

D-AKAP-1, DAKAP1, A-kinase anchor protein 149 kDa, A-kinase anchor protein 1, mitochondrial, AKAP 1

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  WB analysis using 15618-1-AP (same clone as 15618-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15618-1-AP (AKAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 15618-1-AP (same clone as 15618-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 15618-1-AP (AKAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

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  IP experiment of PC-3 using 15618-1-AP (same clone as 15618-1-PBS)

  IP result of anti-AKAP1 (IP:15618-1-AP, 4ug; Detection:15618-1-AP 1:2000) with PC-3 cells lysate 1800ug. This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 15618-1-AP (same clone as 15618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15618-1-AP (AKAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 15618-1-AP (same clone as 15618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15618-1-AP (AKAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 15618-1-AP (same clone as 15618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15618-1-AP (AKAP1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 15618-1-AP (same clone as 15618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15618-1-AP (AKAP1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 15618-1-AP (same clone as 15618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15618-1-AP (AKAP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 15618-1-AP (same clone as 15618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15618-1-AP (AKAP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 15618-1-AP (same clone as 15618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 15618-1-AP (AKAP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 15618-1-AP (same clone as 15618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 15618-1-AP (AKAP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 15618-1-AP (same clone as 15618-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using AKAP1 antibody 15618-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15618-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA AKAP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AKAP1 fusion protein Ag8037
• Vollständiger Name: A kinase (PRKA) anchor protein 1
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 149 kDa
• GenBank-Zugangsnummer: BC000729
• Gene symbol: AKAP1
• Gene ID (NCBI): 8165
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AKAP1 (also termed AKAP149) is a human 149 kDa anchoring protein localized in mitochondria and the endoplasmic reticulum/nuclear envelope (ER-NE) network. It Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment.