AKAP12 Polyklonaler Antikörper

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• KD/KO Validated

AKAP12 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25199-1-PBS

Synonyme

A kinase anchor protein 12, AKAP 12, AKAP 250, AKAP-12, AKAP250

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  WB analysis using 25199-1-AP (same clone as 25199-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25199-1-AP (AKAP12 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 25199-1-AP (same clone as 25199-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25199-1-AP (AKAP12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 25199-1-AP (same clone as 25199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25199-1-AP (AKAP12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 25199-1-AP (same clone as 25199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25199-1-AP (AKAP12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 25199-1-AP (same clone as 25199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25199-1-AP (AKAP12 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 25199-1-AP (same clone as 25199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25199-1-AP (AKAP12 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25199-1-PBS bindet in WB, IHC, Indirect ELISA AKAP12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AKAP12 fusion protein Ag17769
• Vollständiger Name: A kinase (PRKA) anchor protein 12
• Berechnetes Molekulargewicht: 1782 aa, 191 kDa
• Beobachtetes Molekulargewicht: 200-300 kDa
• GenBank-Zugangsnummer: BC157067
• Gene symbol: AKAP12
• Gene ID (NCBI): 9590
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AKAP12, also named as AKAP250, is a 1782 amino acid protein, which contains three AKAP domains. AKAP12 localizes in the cytoplasm and is expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells. AKAP12 as an anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC). AKAP12 expression is known to be suppressed in several human malignancies including breast, prostate, gastric and colon cancers. AKAP12 has three isoforms, namely AKAP12A, AKAP12B and AKAP12C, with molecular weights of 305 kDa, 287 kDa and 250 kDa respectively. (PMID: 30385176, PMID: 15258566)