AKAP13 Polyklonaler Antikörper

AKAP13 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27947-1-PBS

Synonyme

A kinase anchor protein 13, AKAP 13, A-kinase anchor protein 13, ARHGEF13, BRX

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  WB analysis using 27947-1-AP (same clone as 27947-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 27947-1-AP (AKAP13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 27947-1-AP (same clone as 27947-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 27947-1-AP (same clone as 27947-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 27947-1-AP (same clone as 27947-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 27947-1-AP (same clone as 27947-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 27947-1-AP (same clone as 27947-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 27947-1-AP (same clone as 27947-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27947-1-PBS bindet in WB, IHC, Indirect ELISA AKAP13 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AKAP13 fusion protein Ag27486
• Vollständiger Name: A kinase (PRKA) anchor protein 13
• Berechnetes Molekulargewicht: 308 kDa
• Beobachtetes Molekulargewicht: 308 kDa
• GenBank-Zugangsnummer: NM_001270546
• Gene symbol: AKAP13
• Gene ID (NCBI): 11214
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms containing c-terminal dbl oncogene homology (DH) and pleckstrin homology (PH) domains. The DH domain is associated with guanine nucleotide exchange activation for the Rho/Rac family of small GTP binding proteins, resulting in the conversion of the inactive GTPase to the active form capable of transducing signals. The PH domain has multiple functions