AKAP3 Polyklonaler Antikörper

AKAP3 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Kat-Nr. : 13907-1-AP

Synonyme

A kinase anchor protein 3, AKAP 110, AKAP 3, AKAP110, AKAP3, Cancer/testis antigen 82, CT82, Fibrousheathin 1, Fibrousheathin I, FSP95, PRKA3, SOB1, Sperm oocyte binding protein

---

Galerie der Validierungsdaten

• 

  WB analysis using 13907-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13907-1-AP (AKAP3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 13907-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13907-1-AP (AKAP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13907-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13907-1-AP (AKAP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 13907-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AKAP3 antibody (13907-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 13907-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AKAP3 antibody (13907-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 9 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

13907-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA AKAP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AKAP3 fusion protein Ag4885
• Vollständiger Name: A kinase (PRKA) anchor protein 3
• Berechnetes Molekulargewicht: 95 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC047535
• Gene symbol: AKAP3
• Gene ID (NCBI): 10566
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AKAP3 is a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. AKAP3 is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. AKAP3 is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.

Publikationen

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; FSIP1 binds HER2 directly to regulate breast cancer growth and invasiveness.; Authors - Tong Liu
mouse	WB	Proc Natl Acad Sci U S A; SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice.; Authors - Haruhiko Miyata
mouse	IF	Development; The FOXJ1 target Cfap206 is required for sperm motility, mucociliary clearance of the airways and brain development; Authors - Anja Beckers
mouse	WB,IF	J Genet Genomics; Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice; Authors - Yaling Wang
mouse	IF	Development; The FOXJ1 target Cfap206 is required for sperm motility, mucociliary clearance of the airways and brain development.; Authors - Anja Beckers
pig	WB	Front Cell Dev Biol; Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa.; Authors - Yongjie Xu