AKAP4 Polyklonaler Antikörper

AKAP4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 24986-1-AP

Synonyme

A kinase anchor protein 4, A kinase anchor protein 82 kDa, AKAP 4, AKAP 82, AKAP4, AKAP82, CT99, FSC1, hAKAP82, HI, p82, PRKA4

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  WB analysis of human testis using 24986-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 24986-1-AP (AKAP4 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 24986-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24986-1-AP (AKAP4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 24986-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24986-1-AP (AKAP4 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 2 publications below

Produktinformation

24986-1-AP bindet in WB, IHC, IF, ELISA AKAP4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AKAP4 fusion protein Ag21762
• Vollständiger Name: A kinase (PRKA) anchor protein 4
• Berechnetes Molekulargewicht: 854 aa, 94 kDa
• Beobachtetes Molekulargewicht: 82 kDa
• GenBank-Zugangsnummer: BC126252
• Gene symbol: AKAP4
• Gene ID (NCBI): 8852
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AKAP4, also named as A-kinase anchor protein 82 kDa, is a 854 amino acid protein, which belongs to the AKAP110 family. AKAP4 localizes to the principle piece of the sperm flagellum and is only expressed in round spermatids. AKAP4 is a major structural component of sperm fibrous sheath and plays a role in sperm motility.

Publikationen

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; FSIP1 binds HER2 directly to regulate breast cancer growth and invasiveness.; Authors - Tong Liu
	WB	Front Cell Dev Biol; Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa.; Authors - Yongjie Xu
human	IF	Andrologia; Successful outcomes of intracytoplasmic sperm injection-embryo transfer using ejaculated spermatozoa from two Chinese asthenoteratozoospermic brothers with a compound heterozygous FSIP2 mutation.; Authors - Yuan Yuan
mouse	IF	Life Sci Alliance; Genetic mutation of Cep76 results in male infertility due to abnormal sperm tail composition; Authors - Brendan J Houston
mouse	WB	Sci China Life Sci; Adenylate kinase phosphate energy shuttle underlies energetic communication in flagellar axonemes; Authors - Huan Wu
	WB	Animals (Basel); Comparative Study on the Sperm Proteomes of Horses and Donkeys; Authors - Hong Ren