AKAP7 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

AKAP7 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 12591-1-AP

Synonyme

A kinase anchor protein 18 kDa, AKAP 18, AKAP 7 isoforms alpha and beta, AKAP18, AKAP7

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Galerie der Validierungsdaten

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  WB analysis of human brain using 12591-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12591-1-AP (AKAP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12591-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12591-1-AP (AKAP7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12591-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12591-1-AP (AKAP7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12591-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12591-1-AP (AKAP7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12591-1-AP

  IP Result of anti-AKAP7 (IP:12591-1-AP, 4ug; Detection:12591-1-AP 1:300) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human brain using 12591-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12591-1-AP (AKAP7 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 12591-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12591-1-AP (AKAP7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 12591-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12591-1-AP (AKAP7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12591-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12591-1-AP (AKAP7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 12591-1-AP

  Immunofluorescent analysis of HepG2 cells, using AKAP7 antibody 12591-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

12591-1-AP bindet in WB, IP, IHC, IF, ELISA AKAP7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AKAP7 fusion protein Ag3297
• Vollständiger Name: A kinase (PRKA) anchor protein 7
• Berechnetes Molekulargewicht: 81 aa, 9 kDa
• Beobachtetes Molekulargewicht: 11 kDa, 18 kDa, 37 kDa
• GenBank-Zugangsnummer: BC016927
• Gene symbol: AKAP7
• Gene ID (NCBI): 9465
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AKAP7, also named as AKAP15 and AKAP18, localize PKA in complexes with CaV1.2 and PLN, respectively. AKAP7 targets the cAMP-dependent protein kinase (PKA) to the plasma membrane, and permits functional coupling to the L-type calcium channel. AKAP7 has short isoform AKAP7 alpha and AKAP7 beta with MW 15-18 kDa; long isoform AKAP7 gamma with MW 37-42 kDa. AKAP7alpha was highly expressed only in brain and weakly in lung lysates from WT animals. This antibody can recognize all the isoforms of AKAP7.

Publikationen

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; Cardiomyocytes from AKAP7 knockout mice respond normally to adrenergic stimulation.; Authors - Jones Brian W BW ; KO Validated
mouse	WB,IF	Elife; Targeted deletion of AKAP7 in dentate granule cells impairs spatial discrimination.; Authors - Brian W Jones ; KO Validated
human, mouse	WB	MBio; Murine AKAP7 Has a 2',5'-Phosphodiesterase Domain That Can Complement an Inactive Murine Coronavirus ns2 Gene.; Authors - Elona Gusho
human	WB	Clin Proteomics; iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy.; Authors - Hongmei Lu

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