AKAP7 Polyklonaler Antikörper

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• KD/KO Validated

AKAP7 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12591-1-PBS

Synonyme

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  WB analysis of human brain using 12591-1-AP (same clone as 12591-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12591-1-AP (AKAP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12591-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 12591-1-AP (same clone as 12591-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12591-1-AP (AKAP7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12591-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 12591-1-AP (same clone as 12591-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12591-1-AP (AKAP7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12591-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 12591-1-AP (same clone as 12591-1-PBS)

  IP result of anti-AKAP7 (IP:12591-1-AP, 4ug; Detection:12591-1-AP 1:300) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12591-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 12591-1-AP (same clone as 12591-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12591-1-AP (AKAP7 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12591-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 12591-1-AP (same clone as 12591-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12591-1-AP (AKAP7 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12591-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 12591-1-AP (same clone as 12591-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12591-1-AP (AKAP7 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12591-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 12591-1-AP (same clone as 12591-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12591-1-AP (AKAP7 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12591-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12591-1-AP (same clone as 12591-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using AKAP7 antibody 12591-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 12591-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12591-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA AKAP7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AKAP7 fusion protein Ag3297
• Vollständiger Name: A kinase (PRKA) anchor protein 7
• Berechnetes Molekulargewicht: 81 aa, 9 kDa
• Beobachtetes Molekulargewicht: 11 kDa, 18 kDa, 37 kDa
• GenBank-Zugangsnummer: BC016927
• Gene symbol: AKAP7
• Gene ID (NCBI): 9465
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AKAP7, also named as AKAP15 and AKAP18, localize PKA in complexes with CaV1.2 and PLN, respectively. AKAP7 targets the cAMP-dependent protein kinase (PKA) to the plasma membrane, and permits functional coupling to the L-type calcium channel. AKAP7 has short isoform AKAP7 alpha and AKAP7 beta with MW 15-18 kDa; long isoform AKAP7 gamma with MW 37-42 kDa. AKAP7alpha was highly expressed only in brain and weakly in lung lysates from WT animals. This antibody can recognize all the isoforms of AKAP7.