AKD2 Polyklonaler Antikörper

AKD2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 27554-1-AP

Synonyme

AK9, Adenylate kinase domain-containing protein 1, Adenylate kinase domain-containing protein 2, AK 9, AKD1

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  WB analysis using 27554-1-AP

  Various lysates lysate were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 27554-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HuH-7 using 27554-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27554-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 27554-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 27554-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 27554-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 27554-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SMMC-7721-Zellen, HepG2-Zellen, HuH-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

27554-1-AP bindet in WB, IHC, ELISA AKD2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AKD2 fusion protein Ag25805
• Vollständiger Name: adenylate kinase domain containing 2
• Berechnetes Molekulargewicht: 221 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC022031
• Gene symbol: AKD2
• Gene ID (NCBI): 221264
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AK9, also named as adenylate kinase 9, belongs to adenylate kinase family, which is a small, ususlly monomeric, enzyme found in every living thing due to its crucial role in energenic metabolism(PMID: 30666486). AK9 is a nucleoside mono- and diphosphate kinase due to its broad phosphatase activity, the local concentration of substrates probably influences catalytic activity(PMID: 24495878). This antibody can detect 45-50 kDa band of AK9(PMID: 23416111).

Publikationen

Species	Application	Title
human	WB	Mol Carcinog; Integrative analyses of N6-methyladenosine-associated single-nucleotide polymorphisms (m6A-SNPs) identify tumor suppressor gene AK9 in lung cancer; Authors - Tingting Hua