AKD2 Polyklonaler Antikörper

AKD2 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27554-1-PBS

Synonyme

AK9, Adenylate kinase domain-containing protein 1, Adenylate kinase domain-containing protein 2, AK 9, AKD1

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  WB analysis using 27554-1-AP (same clone as 27554-1-PBS)

  Various lysates lysate were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

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  WB analysis of SMMC-7721 using 27554-1-AP (same clone as 27554-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

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  WB analysis of HuH-7 using 27554-1-AP (same clone as 27554-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 27554-1-AP (same clone as 27554-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 27554-1-AP (same clone as 27554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 27554-1-AP (same clone as 27554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 27554-1-AP (same clone as 27554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 27554-1-AP (same clone as 27554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27554-1-PBS bindet in WB, IHC, Indirect ELISA AKD2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AKD2 fusion protein Ag25805
• Vollständiger Name: adenylate kinase domain containing 2
• Berechnetes Molekulargewicht: 221 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC022031
• Gene symbol: AKD2
• Gene ID (NCBI): 221264
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AK9, also named as adenylate kinase 9, belongs to adenylate kinase family, which is a small, ususlly monomeric, enzyme found in every living thing due to its crucial role in energenic metabolism(PMID: 30666486). AK9 is a nucleoside mono- and diphosphate kinase due to its broad phosphatase activity, the local concentration of substrates probably influences catalytic activity(PMID: 24495878). This antibody can detect 45-50 kDa band of AK9(PMID: 23416111).