AKR1B10 Polyklonaler Antikörper

AKR1B10 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 18252-1-AP

Synonyme

AKR1B 10, AKR1B11, AKR1B12, Aldo-keto reductase family 1 member B10, Aldose reductase-like

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  WB analysis using 18252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18252-1-AP (AKR1B10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18252-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18252-1-AP (AKR1B10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 18252-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18252-1-AP (AKR1B10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 18252-1-AP

  IP result of anti-AKR1B10 (IP:18252-1-AP, 4ug; Detection:18252-1-AP 1:4000) with HepG2 cells lysate 2000 ug.

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  IHC staining of mouse stomach using 18252-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18252-1-AP (AKR1B10 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 18252-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18252-1-AP (AKR1B10 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, A431-Zellen, HepG2-Zellen, Mauslebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: Maus-Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

18252-1-AP bindet in WB, IHC, IP, ELISA AKR1B10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AKR1B10 fusion protein Ag13042
• Vollständiger Name: aldo-keto reductase family 1, member B10 (aldose reductase)
• Berechnetes Molekulargewicht: 316 aa, 36 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC008837
• Gene symbol: AKR1B10
• Gene ID (NCBI): 57016
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aldo-keto reductase family 1 member B10 (AKR1B10) is a member of the AKR1B subfamily. It is mainly found in cytoplasm, and it is typically expressed in the stomach and intestines. Given that its expression is low or absent in other tissues, AKR1B10 is a potential diagnostic and therapeutic biomarker for various digestive system diseases.

Publikationen

Species	Application	Title
human	WB	J Pharmacol Exp Ther; Mechanistic Analysis of an Extracellular Signal-Regulated Kinase 2-Interacting Compound that Inhibits Mutant BRAF-Expressing Melanoma Cells by Inducing Oxidative Stress.; Authors - Ramon Martinez
mouse,human	WB	J Ethnopharmacol; Berberine directly targets AKR1B10 protein to modulate lipid and glucose metabolism disorders in NAFLD; Authors - Sa Yang ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shinford (Verified Customer) (10-26-2023)

In tissues, we detected bands at different positions, but in cancer cell lines, the bands appeared notably clearer. The optimal concentration was determined to be higher, specifically at 1:1000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cancer cell lines and tumor tissues

AKR1B10 Antibody Western Blot validation (1:1000 dilution) in Cancer cell lines and tumor tissues (Cat no:18252-1-AP)